Dihydrorhodamine 123 (DHR 123) is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. It is not fluorescent until oxidized by ROS to the highly fluorescent product Rhodamine 123 . Neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DHR, rather, these ROS are thought to combine with other cellular components such as cytochrome c oxidase or Fe2+ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123. Formation of Rhodamine 123 can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).
純度:95%
應(yīng)用:A fluorescent probe commonly used for determination of peroxynitrite & other ROSs.
2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
WSP-1 (Washington State Probe-1)是一種含活性二硫化物的熒光探針,特別設(shè)計(jì)用來(lái)檢測(cè)生物樣本和細(xì)胞內(nèi)的硫化氫(H2S)。通過(guò)基于反應(yīng)的熒光開啟策略,WSP-1選擇性且快速與H2S反應(yīng),生成苯丙二硫醇酮(benzodithiolone)和一種熒光化合物(Ex=465nm,Em=515nm)。
WSP-5 (Washington State Probe-5)是一種含活性二硫化物的熒光探針,特別設(shè)計(jì)用來(lái)快速檢測(cè)生物樣本和細(xì)胞內(nèi)的硫化氫(H2S)。通過(guò)基于反應(yīng)的熒光開啟策略,WSP-5選擇性且快速與H2S反應(yīng),產(chǎn)生一種熒光化合物(Ex=502nm,Em=525nm)。與WSP-1相比,WSP-5靶向H2S表現(xiàn)出更快的熒光開啟速率(60 vs. 130-fold)和更靈敏的檢測(cè)極限(47 vs. 60 nM)。